3A4J
arPTE (K185R/D208G/N265D/T274N)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.946, 108.946, 62.884 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.250 |
| R-factor | 0.12867 |
| Rwork | 0.128 |
| R-free | 0.14470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d2j |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.560 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 105798 | |
| <I/σ(I)> | 5.7 | 3.3 |
| Completeness [%] | 93.9 | 63.8 |
| Redundancy | 11 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% PEG3350, 0.2M NANO3, pH7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
