3A4C
Crystal structure of cdt1 C terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.565, 52.565, 96.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.169 - 1.889 |
| R-factor | 0.2125 |
| Rwork | 0.211 |
| R-free | 0.23580 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.889 | 1.889 |
| Rmerge | 0.081 | 0.373 |
| Number of reflections | 11409 | |
| <I/σ(I)> | 67 | 12.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.2 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 3.8M NaCl, 100mM Sodium Citrate, 5mM DTT, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
