3A4A
Crystal structure of isomaltase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-13 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.428, 115.404, 61.606 |
| Unit cell angles | 90.00, 91.19, 90.00 |
Refinement procedure
| Resolution | 23.000 - 1.600 |
| R-factor | 0.15946 |
| Rwork | 0.159 |
| R-free | 0.17426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a47 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.173 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.042 | 0.050 |
| Number of reflections | 87353 | |
| <I/σ(I)> | 25.8 | 13.8 |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 0.042 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 288 | 19% PEG 3350, 0.05M HEPES, 0.2M lithium acetate, 0.2M maltose, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
