3A47
Crystal structure of isomaltase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-17 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9780 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.709, 115.674, 61.837 |
| Unit cell angles | 90.00, 91.33, 90.00 |
Refinement procedure
| Resolution | 26.240 - 1.590 |
| R-factor | 0.17582 |
| Rwork | 0.175 |
| R-free | 0.19730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uok |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.035 | 0.040 |
| Number of reflections | 86661 | |
| <I/σ(I)> | 39.3 | 26.3 |
| Completeness [%] | 97.1 | 95 |
| Redundancy | 4.3 | 0.042 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 288 | 19% PEG 3350, 0.05M HEPES, 0.2 M lithium acetate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
