3A3K
Reversibly bound chloride in the atrial natriuretic peptide receptor hormone-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SBC-2 |
| Wavelength(s) | 0.91950 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.521, 120.521, 160.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.500 |
| R-factor | 0.20711 |
| Rwork | 0.205 |
| R-free | 0.24290 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.237 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.379 |
| <I/σ(I)> | 22 | 3.9 |
| Completeness [%] | 98.3 | 99.7 |
| Redundancy | 8.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
