3A3C

Crystal structure of TIM40/MIA40 fusing MBP, C296S and C298S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-24
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	41.527, 101.837, 109.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.880 - 2.500
R-factor	0.254
Rwork	0.254
R-free	0.31600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zxt
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	15266
<I/σ(I)>		9.5
Completeness [%]	91.3	91.7
Redundancy	12.1	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	100mM K-acetate, 200 mM NH4-acetate, 30% PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K