3A37
Structural insight into the membrane insertion of tail-anchored proteins by Get3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-28 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 115.229, 222.554, 49.291 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.290 - 3.000 |
R-factor | 0.253 |
Rwork | 0.253 |
R-free | 0.28200 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 25728 | |
<I/σ(I)> | 23.2 | 2.5 |
Completeness [%] | 97.8 | 94.6 |
Redundancy | 5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 13.5% PEG 3350, 0.18M tri-sodium citrate, 9% MPD, 90mM Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |