3A35

Crystal structure of LumP complexed with riboflavin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-28
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	46.288, 46.582, 161.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.150 - 1.421
R-factor	0.19632
Rwork	0.192
R-free	0.23443
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ddy
RMSD bond length	0.033
RMSD bond angle	2.896
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.559
High resolution limit [Å]	1.421
Number of reflections	58164

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	20% (w/v) PEG 4000, 0.2M MgCl2, 100mM HEPES pH6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K