3A2K

Crystal structure of TilS complexed with tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-13
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	85.734, 157.396, 208.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.751 - 3.650
R-factor	0.2183
Rwork	0.216
R-free	0.26640
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.650
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.710
High resolution limit [Å]	3.650	3.650
Rmerge		0.220
Number of reflections	31287
<I/σ(I)>	18.2	2
Completeness [%]	96.6	81.1
Redundancy	4.9	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	45mM Na-cacodylate (pH 6.5), 200mM NaCl, 225mM ammonium acetate, 11mM CaCl2, 7.2 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	45mM Na-cacodylate (pH 6.5), 200mM NaCl, 225mM ammonium acetate, 11mM CaCl2, 7.2 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K