3A2K
Crystal structure of TilS complexed with tRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.734, 157.396, 208.177 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.751 - 3.650 |
R-factor | 0.2183 |
Rwork | 0.216 |
R-free | 0.26640 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.650 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.710 |
High resolution limit [Å] | 3.650 | 3.650 |
Rmerge | 0.220 | |
Number of reflections | 31287 | |
<I/σ(I)> | 18.2 | 2 |
Completeness [%] | 96.6 | 81.1 |
Redundancy | 4.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 45mM Na-cacodylate (pH 6.5), 200mM NaCl, 225mM ammonium acetate, 11mM CaCl2, 7.2 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 45mM Na-cacodylate (pH 6.5), 200mM NaCl, 225mM ammonium acetate, 11mM CaCl2, 7.2 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |