3A2F
Crystal Structure of Pyrococcus furiosus DNA polymerase/PCNA monomer mutant complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0, 0.9641, 0.9791, 0.9794, 0.9951 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.354, 90.451, 186.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.550 - 2.670 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.083 | 0.443 |
| Number of reflections | 37495 | |
| <I/σ(I)> | 6.7 | 4.2 |
| Completeness [%] | 98.8 | 89.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M ammonium sulfate, 30%(w/v) PEG 5000, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
