3A2A
The structure of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-02-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 |
| Unit cell lengths | 37.650, 37.650, 137.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.650 - 2.000 |
| R-factor | 0.25259 |
| Rwork | 0.252 |
| R-free | 0.26621 |
| Structure solution method | MIR |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.314 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.650 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.081 | 0.323 |
| Number of reflections | 12915 | |
| <I/σ(I)> | 12 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 289 | 3.2M NaCl, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, temperature 289K |
