3A21
Crystal Structure of Streptomyces avermitilis beta-L-Arabinopyranosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-11-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.983 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.205, 98.916, 181.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.370 - 1.510 |
| R-factor | 0.15792 |
| Rwork | 0.157 |
| R-free | 0.17946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uas 1xyf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.086 | 0.335 |
| Number of reflections | 191711 | |
| <I/σ(I)> | 30.6 | 6.5 |
| Completeness [%] | 98.1 | 91.2 |
| Redundancy | 7.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.7M ammonium sulfate, 1.7% PEG 400, 15% glycerol, 0.1M HEPES, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
