3A1Z
Crystal structure of juvenile hormone binding protein from silkworm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-06-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.863, 114.655, 192.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.490 - 2.590 |
| R-factor | 0.22528 |
| Rwork | 0.222 |
| R-free | 0.28994 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.753 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.099 | 0.489 |
| Number of reflections | 38636 | |
| <I/σ(I)> | 22.2 | 3.1 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 12.3 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25% MPD, 0.05M zinc acetate, 0.1M sodium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
