3A1Y
The structure of archaeal ribosomal stalk P1/P0 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.880, 104.429, 136.223 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.990 - 2.130 |
R-factor | 0.223 |
Rwork | 0.220 |
R-free | 0.26000 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.552 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.210 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.106 | 0.408 |
Number of reflections | 44207 | |
<I/σ(I)> | 12.4 | 2 |
Completeness [%] | 99.1 | 93 |
Redundancy | 6.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MgCl2, 0.1M Na-Acetate trihydrate, 6-10% PEG 6000, pH 4.2-4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |