3A1S

Crystal structue of the cytosolic domain of T. maritima FeoB iron iransporter in GDP form I

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97918, 0.97942, 0.96419, 0.99515
Spacegroup name	P 21 21 2
Unit cell lengths	107.166, 109.723, 46.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.834 - 1.500
R-factor	0.1737
Rwork	0.171
R-free	0.21700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Number of reflections	88234

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	60-64% MPD, 0.1M HEPES, pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K