3A1P
Structure of Ribosome maturation protein RimM and Ribosomal protein S19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.397, 67.491, 62.002 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 45.570 - 2.300 |
| Rwork | 0.197 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DYI AND 2E5L FOR RIMM AND RIBOSOMAL PROTEIN S19 RESPECTIVELY. |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 20948 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M sodium cacodylate, 0.2M sodium acetate trihydrate, 30%[w/v] polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
