3A1K

Crystal structure of Rhodococcus sp. N771 Amidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	92.672, 92.672, 162.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.170
Rwork	0.201
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g3i
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.170	2.170
Number of reflections	37182
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K