3A1D
Crystal structure of the P- and N-domains of CopA, a copper-transporting P-type ATPase, bound with ADP-Mg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 89.956, 89.956, 190.375 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.850 |
R-factor | 0.19379 |
Rwork | 0.192 |
R-free | 0.22563 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b8e |
RMSD bond length | 0.011 |
RMSD bond angle | 1.285 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.129 | 0.483 |
Number of reflections | 63866 | |
<I/σ(I)> | 30.6 | 5.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 283 | pH 5.5, temperature 283K |