3A0E

Crystal Structure of Polygonatum cyrtonema lectin (PCL) complexed with dimannoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 1W2B
Synchrotron site	BSRF
Beamline	1W2B
Detector technology	IMAGE PLATE
Collection date	2008-03-10
Detector	MAR555 FLAT PANEL
Spacegroup name	P 32 2 1
Unit cell lengths	76.660, 76.660, 61.401
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.080 - 2.000
R-factor	0.19
Rwork	0.190
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a0d
RMSD bond length	0.005
RMSD bond angle	1.470
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.080	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.076	0.265
Number of reflections	14437
<I/σ(I)>		8
Completeness [%]	100.0	100
Redundancy	13.9	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0M LiSO4, 2% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K