3A0D

Crystal Structure of Polygonatum cyrtonema lectin (PCL) complexed with monomannoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Detector technology	IMAGE PLATE
Collection date	2008-02-22
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 32 2 1
Unit cell lengths	76.460, 76.460, 61.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.160 - 2.200
R-factor	0.198
Rwork	0.198
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a0c
RMSD bond length	0.005
RMSD bond angle	1.430
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.170	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.349
Number of reflections	10885
<I/σ(I)>		4.5
Completeness [%]	99.8	100
Redundancy	6.2	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.0M LiSO4, 2% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K