3A0B
Crystal structure of Br-substituted Photosystem II complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 130.589, 226.394, 307.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.700 - 3.700 |
| R-factor | 0.302 |
| Rwork | 0.302 |
| R-free | 0.35800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2axt |
| RMSD bond length | 0.033 |
| RMSD bond angle | 3.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.076 | 0.732 |
| Total number of observations | 565579 | |
| Number of reflections | 89037 | |
| <I/σ(I)> | 26.3 | 1.6 |
| Completeness [%] | 89.4 | 54.3 |
| Redundancy | 6.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 1450, 40mM MgSO4, 10mM MgCl2, 25% glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
