3ZZT
Crystal structure of Staphylococcus aureus elongation factor G with a fusidic-acid-resistant mutation F88L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.970, 127.930, 107.440 |
| Unit cell angles | 90.00, 107.86, 90.00 |
Refinement procedure
| Resolution | 47.402 - 2.950 |
| R-factor | 0.2434 |
| Rwork | 0.241 |
| R-free | 0.29370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fnm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.080 | 0.950 |
| Number of reflections | 32339 | |
| <I/σ(I)> | 14.57 | 1.64 |
| Completeness [%] | 90.2 | 72.4 |
| Redundancy | 3.76 | 3.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
