3ZZP
Circular permutant of ribosomal protein S6, lacking edge strand beta- 2 of wild-type S6.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 22.200, 40.800, 68.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.960 |
| R-factor | 0.1129 |
| R-free | 0.13690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.033 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.990 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmerge | 0.060 | 0.650 |
| Number of reflections | 38300 | |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 6 | 4.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.9M AMMONIUM SULPHATE, 0.1M MES PH 6.5, 8% 1,4 DIOXANE |
