3ZZN
5-Mutant (R79W, R151A, E279A, E299A,E313A) Lactate-Dehydrogenase from Thermus thermophillus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-10 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.123, 59.597, 153.076 |
Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
Resolution | 45.470 - 2.900 |
R-factor | 0.1919 |
Rwork | 0.188 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v6m |
RMSD bond length | 0.015 |
RMSD bond angle | 1.704 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.530 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.103 | 0.485 |
Number of reflections | 30826 | |
<I/σ(I)> | 14.25 | 3.05 |
Completeness [%] | 96.8 | 96.8 |
Redundancy | 3.47 | 3.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 5-MUT TT-LDH WAS CRYSTALLIZED AT 10MG/ML BY THE HANGING-DROP VAPOUR-DIFFUSION METHOD, FOLLOWING THE MIXING OF THE PROTEIN AND THE MOTHER-LIQUOR SOLUTIONS AT A 1:1 RATIO. THE MOTHER LIQUOR SOLUTION WAS 0.1 M HEPES BUFFER PH 9, 18-20% PEG 6000, 2 M LICL2, 500 MICROMOLAR ADP. CRYSTALS GREW WITHIN A WEEK AT 4 DEGREES CELSIUS. |