3ZZ0

Crystal structure of ribosomal elongation factor (EF)-G from Staphylococcus aureus with a fusidic acid hyper-sensitivity mutation M16I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-06
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	64.940, 125.460, 106.270
Unit cell angles	90.00, 107.52, 90.00

Refinement procedure

Resolution	46.710 - 2.800
R-factor	0.25
Rwork	0.249
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fnm
RMSD bond length	0.004
RMSD bond angle	0.965
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	3.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.140	0.860
Number of reflections	36516
<I/σ(I)>	7.55	1.85
Completeness [%]	91.1	76.5
Redundancy	3.73	3.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1