3ZYO
Crystal structure of the N-terminal leucine rich repeats and immunoglobulin domain of netrin-G ligand-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 131.330, 131.330, 174.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.600 - 3.100 |
| R-factor | 0.23044 |
| Rwork | 0.229 |
| R-free | 0.26931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zyn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.857 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.130 | 0.870 |
| Number of reflections | 10743 | |
| <I/σ(I)> | 11.2 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M NAH2PO4, 0.1 M KH2PO4, 2M NACL, 0.1 M MES, PH 6.5 |
