3ZYN
Crystal structure of the N-terminal leucine rich repeats of Netrin-G Ligand-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.100, 57.260, 100.430 |
| Unit cell angles | 90.00, 117.76, 90.00 |
Refinement procedure
| Resolution | 52.940 - 3.200 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2id5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.140 | 0.580 |
| Number of reflections | 13296 | |
| <I/σ(I)> | 8.2 | 2.2 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.8 M SUCCINIC ACID PH 7.0. |
