3ZY6
Crystal structure of POFUT1 in complex with GDP-fucose (crystal-form-II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 90 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.583, 38.156, 68.101 |
| Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
| Resolution | 70.480 - 1.910 |
| R-factor | 0.20314 |
| Rwork | 0.202 |
| R-free | 0.23733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zy2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.375 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 39386 | |
| <I/σ(I)> | 9.27 | 1.97 |
| Completeness [%] | 98.9 | 96.5 |
| Redundancy | 4.37 | 3.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
