3ZXT
Dimeric structure of DAPK-1 catalytic domain in complex with AMPPCP- Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.407, 76.578, 106.892 |
| Unit cell angles | 90.00, 92.01, 90.00 |
Refinement procedure
| Resolution | 106.830 - 2.650 |
| R-factor | 0.21947 |
| Rwork | 0.216 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w4j |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.756 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.710 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 39321 | |
| <I/σ(I)> | 10.2 | 2.5 |
| Completeness [%] | 99.8 | 97.4 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH7.0, 0.1M MGCL2, 15% PEG4000 |
