3ZXE
Crystal structure of Human Galectin-7 in complex with a galactose- benzylphosphate inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.287, 53.459, 138.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.340 - 1.670 |
| R-factor | 0.20595 |
| Rwork | 0.205 |
| R-free | 0.23633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.070 | 0.130 |
| Number of reflections | 31402 | |
| <I/σ(I)> | 20.4 | 9.6 |
| Completeness [%] | 92.9 | 84.4 |
| Redundancy | 4.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M BISTRISPROPANE, 20% PEG3350, pH 8.5 |
