3ZX7
Complex of lysenin with phosphocholine
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.110, 98.110, 184.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.700 - 2.840 |
| R-factor | 0.2255 |
| Rwork | 0.224 |
| R-free | 0.26400 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.100 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 12994 | |
| <I/σ(I)> | 29 | 4.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 22.1 | 23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 1.8 M AMMONIUM DI-HYDROGEN PHOSPHATE, 1.0 M SODIUM ACETATE PH 4.5 |
