3ZWG

Crystal structure of the pore-forming toxin FraC from Actinia fragacea (form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 3 1 2
Unit cell lengths	118.770, 118.770, 431.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.969 - 3.000
R-factor	0.2065
Rwork	0.204
R-free	0.25010
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.207
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	66.140
High resolution limit [Å]	3.000
Rmerge	0.130
Number of reflections	68444
<I/σ(I)>	8.9
Completeness [%]	97.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				4M SODIUM FORMATE