3ZWF
Crystal structure of Human tRNase Z, short form (ELAC1).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.520, 73.790, 73.660 |
| Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
| Resolution | 72.810 - 1.700 |
| R-factor | 0.18776 |
| Rwork | 0.187 |
| R-free | 0.21015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL DETERMINED FROM SIRAS EXPERIMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.490 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.570 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.040 |
| Number of reflections | 67111 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 97.0 | 95.3 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15% PEG 3350 0.2M NA NITRATE, pH 7 |
