3ZVR
Crystal structure of Dynamin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-17 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 178.450, 191.610, 60.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.805 - 3.100 |
| R-factor | 0.2153 |
| Rwork | 0.212 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2AKA CHAIN B PDB ENTRY 2DYN CHAIN B PDB ENTRY 3LJB CHAIN B |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.800 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.070 | 0.880 |
| Number of reflections | 18735 | |
| <I/σ(I)> | 15.4 | 1.9 |
| Completeness [%] | 96.7 | 66.2 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 52.5 MM TRIS.HCL PH 7.7, 175 MM NACL, 32.5 MM NANO3, 20 % V/V PEG 400, 0.97 MM BETA-MERCAPTOETHANOL, 31.9 MICROM DYN1 G397D DELTA PRD |
