3ZVL
The structural basis for substrate recognition by mammalian polynucleotide kinase 3' phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.750, 63.500, 67.900 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 33.933 - 1.650 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.026 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.930 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.570 |
| Number of reflections | 39354 | |
| <I/σ(I)> | 13.1 | 2.3 |
| Completeness [%] | 90.1 | 58.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.05 M NACACODYLATE PH 6.5, 0.2 M AMMONIUM ACETATE, 0.01 M MG ACETATE, 30 % (W/V) PEG 8000. |
