3ZVK
Crystal structure of VapBC2 from Rickettsia felis bound to a DNA fragment from their promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.780, 92.333, 150.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.420 - 2.500 |
| R-factor | 0.1745 |
| Rwork | 0.172 |
| R-free | 0.21720 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.9.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.560 |
| Number of reflections | 39577 | |
| <I/σ(I)> | 17.4 | 3.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 12% PEG4000, 4% PEG400, 100MM MES PH6.5 | ||
| 1 | 6.5 | 12% PEG4000, 4% PEG400, 100MM MES PH6.5 |
