3ZVI
Methylaspartate ammonia lyase from Clostridium tetanomorphum mutant L384A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-08 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.621, 109.863, 110.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.030 - 1.900 |
| R-factor | 0.16252 |
| Rwork | 0.160 |
| R-free | 0.20995 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kcz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.140 | 0.640 |
| Number of reflections | 61377 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 94.2 | 87.2 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 14-17 % PEG6000, MES PH 6.0, 100 MM MGCL2 |
