3ZUX
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 73.330, 73.330, 163.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.640 - 2.200 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.22860 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.860 |
| Number of reflections | 23427 | |
| <I/σ(I)> | 8.8 | 1.2 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 4.32 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 50 MM SODIUM CITRATE PH 4.5, 70 MM NACL, 22-24% PEG400. |
