3ZUO
OMCI in complex with leukotriene B4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-07-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.756, 112.789, 62.394 |
| Unit cell angles | 90.00, 101.89, 90.00 |
Refinement procedure
| Resolution | 61.060 - 1.860 |
| R-factor | 0.2209 |
| Rwork | 0.220 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zui |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.790 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.030 | 0.500 |
| Number of reflections | 46060 | |
| <I/σ(I)> | 17.7 | 2.1 |
| Completeness [%] | 96.9 | 82.6 |
| Redundancy | 2.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.4 | 21% PEG 4000, 0.2 M SODIUM ACETATE, TRIS 0.1 M PH 8.4. |
