3ZUG
E268D mutant of FAD synthetase from Corynebacterium ammoniagenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 133.545, 133.545, 133.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.050 |
| R-factor | 0.19701 |
| Rwork | 0.195 |
| R-free | 0.22925 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.060 | 0.510 |
| Number of reflections | 49927 | |
| <I/σ(I)> | 34.4 | 7.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.7 | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5 M LI2SO4, 0.1 M HEPES PH 7.5 |
