3ZT9
The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-25 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.000, 46.920, 87.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.476 - 1.750 |
| R-factor | 0.1538 |
| Rwork | 0.152 |
| R-free | 0.18860 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.480 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 19966 | |
| <I/σ(I)> | 10.5 | 3.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1 UL PROTEIN, 1 UL CRYSTALLANT, 1 ML WELL, 14 % PEG 3350, 0.2 M NACL, 0.1 M BIS-TRIS PH 5.5. |
