3ZT7
GlgE isoform 1 from Streptomyces coelicolor with beta-cyclodextrin and maltose bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.270, 113.380, 315.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.800 - 2.500 |
| R-factor | 0.19305 |
| Rwork | 0.192 |
| R-free | 0.22117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zst |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.447 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.420 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.140 | 0.690 |
| Number of reflections | 140547 | |
| <I/σ(I)> | 10 | 2.3 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
