3ZT5
GlgE isoform 1 from Streptomyces coelicolor with maltose bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-10 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.820, 113.570, 315.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.010 - 2.090 |
R-factor | 0.20487 |
Rwork | 0.204 |
R-free | 0.22757 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zst |
RMSD bond length | 0.015 |
RMSD bond angle | 1.440 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.890 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.120 | 0.430 |
Number of reflections | 236917 | |
<I/σ(I)> | 12 | 3.2 |
Completeness [%] | 99.5 | 96.7 |
Redundancy | 7.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.0 |