3ZST
GlgE isoform 1 from Streptomyces coelicolor with alpha-cyclodextrin bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.240, 113.240, 314.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.540 - 2.300 |
| R-factor | 0.17401 |
| Rwork | 0.173 |
| R-free | 0.20132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEMET DERIVATIVE MODEL SOLVED BY MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.529 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.350 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.330 |
| Number of reflections | 85772 | |
| <I/σ(I)> | 23.6 | 7.9 |
| Completeness [%] | 93.7 | 66 |
| Redundancy | 14.3 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
