3ZRH

Crystal structure of the Lys29, Lys33-linkage-specific TRABID OTU deubiquitinase domain reveals an Ankyrin-repeat ubiquitin binding domain (AnkUBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-29
Spacegroup name	P 21 21 21
Unit cell lengths	60.400, 72.150, 133.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.712 - 2.230
R-factor	0.2035
Rwork	0.200
R-free	0.24780
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.413
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX ((PHENIX.REFINE: DEV_764))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.320	2.350
High resolution limit [Å]	2.230	2.230
Rmerge	0.080	0.490
Number of reflections	29095
<I/σ(I)>	12.1	2.7
Completeness [%]	100.0	100
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.9		150 MM NACL, 100 MM NAOAC, 5 MM MGCL2, 50 MM MES [PH 5.9]