3ZRG
Crystal structure of RxLR effector PexRD2 from Phytophthora infestans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.450, 52.920, 53.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.700 - 1.750 |
| R-factor | 0.19204 |
| Rwork | 0.189 |
| R-free | 0.24550 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.447 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 12601 | |
| <I/σ(I)> | 26.3 | 9.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 15.8 | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.6 M AMMONIUM SULPHATE, 0.1 M TRIS.HCL PH 7.5 AND 0.2 M AMMONIUM BROMIDE |
