3ZQF
Structure of Tetracycline repressor in complex with antiinducer peptide-TAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH MX-225 |
| Spacegroup name | I 41 |
| Unit cell lengths | 68.650, 68.650, 182.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.180 - 2.560 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ns8 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.366 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.710 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.050 | 0.780 |
| Number of reflections | 13467 | |
| <I/σ(I)> | 17.78 | 1.53 |
| Completeness [%] | 99.4 | 98.3 |
| Redundancy | 4.21 | 4.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | RESERVOIR: 3 M NACL, 0.1 M BIS-TRIS, PH 6. |
