3ZQ6
ADP-ALF4 COMPLEX OF M. THERM. TRC40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.948, 49.768, 147.363 |
| Unit cell angles | 90.00, 115.04, 90.00 |
Refinement procedure
| Resolution | 41.206 - 2.107 |
| R-factor | 0.1755 |
| Rwork | 0.173 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2woj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.110 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 77439 | |
| <I/σ(I)> | 19.5 | 1.7 |
| Completeness [%] | 96.3 | 51.9 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 34% PENTAERYTHRITOL PROPOXYLATE, 50 MM HEPES PH 7.0 AND 25 MM KCL |
