3ZQ5
Structure of the Y263F mutant of the cyanobacterial phytochrome Cph1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.570, 95.150, 73.580 |
| Unit cell angles | 90.00, 99.67, 90.00 |
Refinement procedure
| Resolution | 33.860 - 1.950 |
| R-factor | 0.17578 |
| Rwork | 0.174 |
| R-free | 0.21765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vea |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.388 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Rmerge | 0.080 | 0.940 |
| Number of reflections | 53522 | |
| <I/σ(I)> | 11.1 | 1.4 |
| Completeness [%] | 99.8 | 94.1 |
| Redundancy | 3.37 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 10 MG / ML PROTEIN, 1.9 M SODIUM ACETATE, 0.4 M MES PH 7.2. |
