3ZO6

Crystal structure of Bacillus pseudofirmus OF4 mutant ATP synthase c12 ring.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-02
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	90.220, 114.550, 137.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.354 - 4.104
R-factor	0.2777
Rwork	0.275
R-free	0.33540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x2v
RMSD bond length	0.004
RMSD bond angle	1.072
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	4.200
High resolution limit [Å]	4.100	4.100
Rmerge	0.400
Number of reflections	11501
<I/σ(I)>	7.42	1.3
Completeness [%]	98.4	81
Redundancy	3.57	3.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9		pH 9.0